eISSN: 3023-6940
  • Home
  • Evaluation of the Effect of Frozen Ejaculate and FrozenTesticular Sperm Results on Inrtacytoplasmic Sperm Injection (ICSI) Parameters

Original Research

Evaluation of the Effect of Frozen Ejaculate and FrozenTesticular Sperm Results on Inrtacytoplasmic Sperm Injection (ICSI) Parameters


1 İstanbul Medipol Üniversitesi, Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Histoloji ve Embriyoloji AD

2 İstanbul Medipol Üniversitesi, Rejeneratif ve Restoratif Tıp Araştırmaları Merkezi, REMER

3 Florence Nightingale Hastanesi, Tüp Bebek Merkezi


DOI : 10.33719/yud.531645
New J Urol. 2019; 14 (1): 01-06

Abstract

Objective:The aim of the present study is to investigate the effect of frozen ejaculate and frozen testicular sperm on ICSI results.

Materials and Methods: 36 infertile couples that underwent an ICSI cycle and that the semen samples were frozen between 11.10.2016-12.09.2017 in were included in the study. Ejaculate sperm cryo- preservation was performed in 14 patients and mi- cro TESE sperm cryopreservation was performed in 22 patients.ICSI results (fertilization rate, embryo development rate and pregnancy rates) of the two groups were compared.

Results: There were no statistically significant difference between total motility and viability rates before and after freezing in ejaculate and testicular sperm extraction (TESE) sperm (> 0.05). When ICSI parameteres were compared nostatistically signifi- cant difference were observed between fertilization rates, implantation rates and embryo development rates (> 0.05). But pregnancy rates were found sta- tistically significantly higher in frozen ejaculate group than frozen testicular sperm group (<0.05).

Conclusion: Although sperm cryopreservation has been used in ICSI treatments for many years, re- search and debate on the effectiveness of the method continues. According to our results frozen testicular sperm had no adverse effect on ICSI parameteres but we analyzed a significant decrease in pregnancy rates. This may be partially explained by the role of sperm in the implantation process. In order to dem- onstrate this effect and to elucidate the mechanisms involved in the implantation process, molecular studies with larger patient groups are needed.

Keywords: Cryopreservation, Sperm, TESE, ICSI


Abstract

Objective:The aim of the present study is to investigate the effect of frozen ejaculate and frozen testicular sperm on ICSI results.

Materials and Methods: 36 infertile couples that underwent an ICSI cycle and that the semen samples were frozen between 11.10.2016-12.09.2017 in were included in the study. Ejaculate sperm cryo- preservation was performed in 14 patients and mi- cro TESE sperm cryopreservation was performed in 22 patients.ICSI results (fertilization rate, embryo development rate and pregnancy rates) of the two groups were compared.

Results: There were no statistically significant difference between total motility and viability rates before and after freezing in ejaculate and testicular sperm extraction (TESE) sperm (> 0.05). When ICSI parameteres were compared nostatistically signifi- cant difference were observed between fertilization rates, implantation rates and embryo development rates (> 0.05). But pregnancy rates were found sta- tistically significantly higher in frozen ejaculate group than frozen testicular sperm group (<0.05).

Conclusion: Although sperm cryopreservation has been used in ICSI treatments for many years, re- search and debate on the effectiveness of the method continues. According to our results frozen testicular sperm had no adverse effect on ICSI parameteres but we analyzed a significant decrease in pregnancy rates. This may be partially explained by the role of sperm in the implantation process. In order to dem- onstrate this effect and to elucidate the mechanisms involved in the implantation process, molecular studies with larger patient groups are needed.

Keywords: Cryopreservation, Sperm, TESE, ICSI