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Original Research

Comparison of the effects of sperm selection methods on assisted reproduction outcomes in male infertility


1 Üsküdar University Faculty of Medicine, Department of Obstetrics and Gynecology, Istanbul, Turkey
2 Medipol University Medipol Mega  Hospital, Department of in Vitro Fertilization, Istanbul, Turkey


DOI : 10.33719/yud.2023;18-2-1247568
New J Urol. 2023;18(2):166-172

ABSTRACT

Objective: To compare physiological intracytoplasmic sperm injection (PICSI) and the hypoosmotic swelling test (HOST) sperm selection techniques in terms of embryo development, implantation rate and live birth rates in cases of severe or moderate oligoastheneospermia.

Material and Methods: The electronic material and files of cases admitted to the Medipol University IVF Centre between 2013 and 2022 were analyzed retrospectively. This research included a total of 143 cases with moderate or severe oligoasthenospermia,  as a sperm selection technique, PICSI has been used in 80 cases and HOST has been  used in 63 cases. Cases of severe or moderate oligoasthenospermia as defined by the World Health Organisation (WHO) are included in this study. The demographic parameters of both groups, including female age, paternal age, duration of infertility, anti-Mullerian hormone (AMH), body mass index (BMI), endometrial thickness, and the number of prior fertility attempts, were analyzed. Implantation rate, pregnancy loss, and rates of live births were compared between two groups.

Results: Female age, paternal age, AMH, endometrial thickness, BMI, and the number of prior fertility attemps were similar between the groups. There were not any significant differences between the groups in terms of fertilization rate (10.2±6.9 vs 9.1±6.4, p=0.345), TQ-GQ blastocyst development (2.4±2.4 vs 2.6±1.4 , p=0.097), and the number of embryos transferred (1.6±0.5 vs 1.8±0.4, p= 0.141). Although the implantation rate is significantly higher in the HOST group than PICSI group (p=0.043), the live birth rates were similar. 

Conclusion: There were no any differences in embryo development, pregnancy loss, and live birth rates comparing the two methods, however, the HOST group had a higher implantation rate.

Keywords: PICSI, HOST, Male infertility 


ABSTRACT

Objective: To compare physiological intracytoplasmic sperm injection (PICSI) and the hypoosmotic swelling test (HOST) sperm selection techniques in terms of embryo development, implantation rate and live birth rates in cases of severe or moderate oligoastheneospermia.

Material and Methods: The electronic material and files of cases admitted to the Medipol University IVF Centre between 2013 and 2022 were analyzed retrospectively. This research included a total of 143 cases with moderate or severe oligoasthenospermia,  as a sperm selection technique, PICSI has been used in 80 cases and HOST has been  used in 63 cases. Cases of severe or moderate oligoasthenospermia as defined by the World Health Organisation (WHO) are included in this study. The demographic parameters of both groups, including female age, paternal age, duration of infertility, anti-Mullerian hormone (AMH), body mass index (BMI), endometrial thickness, and the number of prior fertility attempts, were analyzed. Implantation rate, pregnancy loss, and rates of live births were compared between two groups.

Results: Female age, paternal age, AMH, endometrial thickness, BMI, and the number of prior fertility attemps were similar between the groups. There were not any significant differences between the groups in terms of fertilization rate (10.2±6.9 vs 9.1±6.4, p=0.345), TQ-GQ blastocyst development (2.4±2.4 vs 2.6±1.4 , p=0.097), and the number of embryos transferred (1.6±0.5 vs 1.8±0.4, p= 0.141). Although the implantation rate is significantly higher in the HOST group than PICSI group (p=0.043), the live birth rates were similar. 

Conclusion: There were no any differences in embryo development, pregnancy loss, and live birth rates comparing the two methods, however, the HOST group had a higher implantation rate.

Keywords: PICSI, HOST, Male infertility 

Resources

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